To study the content of total terpene lactones in Ginkgo biloba L. leaves from various area and varieties in Anhui province.Methods:Ginkgo biloba L. leaves were collected from six growth area in Anhui province, and the content of total terpene lactones were measured.Results:The contents of total terpene lactones were different in Ginkgo biloba L. leaves from various area and varieties.Conclusion:The result could provide scientific basis for rational planting of Ginkgo biloba L. in different areas of Anhui province.