To explore the clinical diagnosis value and feasibility of TCM Five Inspections on ovarian function using transvaginal ultrasound. Method: Eighty infertile females in follicular phase of natural menstrual cycle, had the mean ovarian diameter (MOD) and antral follicle count (AFC) measured using transvaginal ultrasound, and evaluated by five inspections. Compared the results of two evaluation methods.Results:The diagnosis results of the Five Inspections had a linear correlation with MOD and AFC results (P<0.05), indicating the Five Inspections on ovarian function assessment is statistically significant, the ROC（receiver operating characteristic curves) showed the asymptotic 95% confidence interval (0.546,0.838), not including 0.5, indicating that the Five Inspections had some application value on ovarian function assessment.Conclusion:TCM Five Inspections can be used in the diagnosis of the ovarian function.