To explore the effect of Shaoyao Shujin tablet on the cartilage pathology, PGE₂, TNF-α and the level of PⅡCP of the rat with knee joint inflammation. Methods:Sixty SD rats were divided into the 6 groups: fake surgery group, model group, control group, high-dose test group, mid-dose test group and the low-dose test group. The Anterior Cruciate Ligament Transection, ACLT were given to rats in the latter 5 groups to establish knee osteoarthritis model. Abdominal aortic blood was separated and the surgery side of the knee was taken after 4 weeks of drug administration. The serum with PGE₂, TNF-α and PⅡCP were checked out after separation. Results:The morphological changes of articular cartilage under optical microscope indicated that the model was successful. The concentration of PGE₂ and PⅡCP showed statistically significant differences between the test groups and the model group. And the concentration of TNF-α in the test groups had a decreasing trend compared with that in the model group. Conclusion: Shaoyao Shujin tablet can be anti-inflammatory, delay the degeneration of the knee osteoarthritis articular cartilage and so as to protect the articular cartilage.