To investigate different perspectives of the triple-energizer theory, provide guidance for the study of the clinical practice of chronic nephritis, and furthermore clarify the therapeutic mechanism of “smoothing Shaoyang” on the treatment of chronic nephritis. Methods: Generalize the characteristics and the essences of the theory of triple-energizer from the angles of three aspects namely the conception, qi-energizing, and its relevance to kidney. By means of the method of “smoothing Shaoyang” that is based on the theory of triple energizer, the anti-nephritis 3rd prescription formula was established and clinical and experimental methodologies were designed as well to investigate its therapeutic mechanism. Results: Triple-energizer (also called Tri-jiao) is presumed as a membranous, meshwork-like tissue theoretically, which is functioning as balance regulation of zang and fu, the pathway of material substance transportation. It plays a crucial role in qi-energizing by means of facilitating water and the essence of grain assimilating, warming and exploring along the pathway, and cleaning the water channel in human body. Almost all of its physiological functions are related closely to kidney; when there is malfunction in the kidney, the basic treatment should be smoothing Shaoyang and mediate triple-energizer. The overall effective percentage for the anti-nephritis 3rd prescription formula is 87%, with the complete remission rate being 32%. At the same time, long term administration of the anti-nephritis 3rd prescription formula can abate urea protein, hematuria, elevate the level of plasma protein, participate to regulate immune system, down-regulate the blood lipid and blood pressure, and ameliorate thrombin and blood coagulation. The anti-nephritis 3rd prescription formula has also been observed to alleviate the pathological alterations in the total of four types of animal nephritis, participate in the regulation of cytokine regulating network, and inhibit and reverse the phenotypic transform in renal mesangial cell and tubular mesenchyma cell. As a result, it proved to mitigate nephro-tubular damage and delayed the pathological process of glomerulus sclerosis. Conclusion: The anti-nephritis 3rd prescription formula, which has been proved to be effective in the treatment of nephritis by means of smoothing Shaoyang of triple-energizer, may possess a very valuable scientific importance and application perspective.