To study the application effect of multimedia combined with problem-based learning(PBL)teaching mode in internship of TCM surgery. Methods: Eighty SHUTCM undergraduates who were having internship in TCM surgery were divided into two groups randomly. The teaching quality of the two groups was evaluated under different teaching mode. Students in the study group were given multimedia combined with PBL teaching mode, and the control group applied traditional teaching mode. Students in both groups took close-book examination and learning effect questionnaire survey as the way to evaluate. Results: After comparing multimedia combined with PBL teaching mode with the traditional teaching mode, it was found that the examination results of students in the study group were better than those of the control group (P<0.01).In addition, higher satisfaction was shown in the learning effect questionnaire of the students in study group (P<0.01). Conclusion: The study clarifies the advantages of the multimedia combined with PBL teaching mode applied in internship teaching of TCM surgery, and provides a new thought for its teaching mode reform.