To investigate the effects of Ginsenoside Re on voltage-dependent sodium current and potassium current in rat ventricular myocytes, and to investigate the mechanism of antiarrhythmia . Methods: The effect of Ginsenoside Re on sodium current(INa), transient outward potassium current(Ito) and inwardly rectifie- d potassium current（Ik1）were assessed by applying whole-cell patch clamp technique in single cardiac myocytes. Results: Ginsenoside Re (10 ,100μmol·L-1)) was shown to inhibit the altitude of INa and the inhibition ratio was 25.5±5.7%（n=3）and 46.2±18%（n=4）. It was shown a little inhibition by Ginsenoside Re (100μmol·L-1)) and its effects were reversible completely after washout. The inhibition ratio of Ito was 13±9%（n=3）in 100μmol·L-1). Ginsenoside Re (10 ,100μmol·L-1)) inhibited the altitude of Ik1 and the inhibition ratio was 16.3±8.7%（n=5）and 35.4±11.2%（n=5）. Conclusion: Ginsenoside Re can inhibit sodium current and potassium currents to produce antiarrhythmic effect.