To observe the clinical curative effect of Duhuojisheng decoction combined with acupuncture in the treatment of osteoarthritis of the knee. Methods: Sixty patients were randomly divided into 2 groups. The 30 cases in treatment group were treated with Duhuojisheng decoction combined with acupuncture treatment. The 30 cases in the control group received acupuncture and western medicine. One treatment course lasted 10 days and clinical evaluation was made after 2 courses. Therapeutic effect, Lysholm Knee Score, LKSS and adverse reactions after the treatment were observed. Results: The total effective rates of the treatment group and the control group were respectively 86.7% and 63.3% (P<0.05). Comparing the changes of LKSS, at the end of treatment, the LKSS of both groups were higher than that before the treatment. The difference was statistically significant (P<0.05). After 10 days (times) of treatment, the LKSS of the treatment group was lower than that of the control group (P>0.05).At the end of the whole treatment, the LKSS of the treatment group was higher than that the control group. The difference was statistically significant (P<0.05). Adverse reactions of the treatment group was significantly lower than that of the control group. Conclusion: Duhuojisheng decoction combined with acupuncture in the treatment of knee osteoarthritis would improve blood circulation, relieve muscle spasm, pain and promote recover of knee joint function.