To investigate effects of Xuefu Zhuyu Decoction(XFZYD) on levels of Nitric oxide (NO) released from Human Umbilical Vein Endothelial cells. Methods: Endothelial cells HUVEC-12 were incubated with blank serum or XFZYD containing serum (XFZYD-CS) at different concentrations and different times. Thereafter, the NO concentrations in the culture supernatant liquid were determined. Results: Compared with the control group, XFZYD-CS has a moderate stimulatory effect on NO production of this endothelial cell line in a period of time. Conclusion: Xuefu Zhuyu Decoction moderately increases the endothelial NO concentration to promote angiogenesis without causing overgrown blood vessels.