To observe the effect of Qisheng mixture on immunity of non-small cell lung cancer which accepted NP treatment. Methods：One hundred and twenty cases of non-small cell lung cancer patients admitted to our hospitals from March 2005 to September 2013 were divided into two groups according to the different treatment methods，with 60 cases in each group. Both groups gave patients chemotherapy regimens NP，and treatment group gave patients extra Qisheng mixture after each session of chemotherapy. White blood cell count，immunity circumstances and granulocyte colony-stimulating factor dosage were compared between the two groups before and after chemotherapy as well as the common symptoms scores after chemotherapy (colds，ulcers，headaches，vomiting，diarrhea) were observed. Results：The white blood cell count of two groups were both less than those before the treatment (P<0.05). At the second，fourth and sixth courses，the white blood cell count in the control group therapy were higher than the treatment group(P<0.05).The dosage of the G-CSF in the treatment was less than that of the control group(P<0.05).Treatment group symptom scores were lower than those of the control group (P<0.05).Conclusion：Qisheng mixture group can effectively improve the immunity of non-small cell lung cancer patients who accepted NP treatment，and it is recommended for clinical application.