To explore the effects of combination of Herba Epimedii and Fructus Ligustri Lucidi on biochemical indicators in glucocorticoid-induced osteoporosis rats. Methods： A total of 60 SD rats were randomly divided into 6 groups：normal control group，model control group，Herba Epimedii group，Fructus Ligustri Lucidi group，Herba Epimedii and Fructus Ligustri Lucidi mixture group，and positive drug group. All rats received intramuscular injection of dexamethasone (1mg/kg，twice a week) for eight weeks to establish osteoporosis model (except those in the control group). Rats in treatment groups were treated with intragastric administration of traditional Chinese herbal decoction or positive drug respectively. Eight weeks later，serum biochemical indexes，including osteocalcin(BGP)，calcium(Ca)，phosphorus(P)，alkaline phosphatase(AKP)，tartrate-resistant acid phosphatase(StrACP)，and the content of bone Ca and bone P were tested. Results： Serum AKP and StrACP activity and the concentration of serum P in model group were significantly higher than that of the normal control group，while the content of serum BGP，serum Ca and bone Ca were obviously lower. Compared with the model group，serum AKP and StrACP activity significantly decreased and serum BGP content increased significantly in each administration group，and serum and bone Ca and P metabolish disorders were corrected in varying degrees. Conclusion： Combination of Herba Epimedii and Fructus Ligustri Lucidi can significantly improve biochemical indicators and effectively correct abnormality of bone metabolism in glucocorticoid-induced osteoporosis rats，thus plays an important role in anti-osteoporosis.