To determine the contents of the moisture, total ash, water-soluble extract and impurities content of Vignae Semen produced in different areas. Methods： The contents were determined according to water determination method (drying) in Appendix IX H, ash detection in Appendix IX K, extractives determination in Appendix XA and impurities determination in Appendix IXA of the 2010 edition of “Chinese Pharmacopoeia”. Results： The average moisture content of the 12 batches of commercially available Vignae Semen was 11.96%, and the highest was 13.64%, and the lowest was 10.12%. The total ash average content was 3.90%, and the highest was 4.36%, and the lowest was 3.61%. The average content of water-soluble extract was 20.73%, and the highest was 24.32% and the lowest was18.16%. The average content of impurities was 0.90%, and the highest was 1.11% and the lowest was 0.67%. Conclusion： These data provide the quality standards basis for further establishment and improvement of Vignae Semen.