To investigate efficacy and toxicity of Fuzi water extract for control and treatment of yang deficiency of spleen and kidney in rats.Methods：Establish rat model of syndrome of yang deficiency of spleen and kidney by administering 15% rhubarb powder suspension to rat for 9 days, 8.9g·kg-1·d-1 everyday and then making it swim in cold water until stressed out. The prevention group was establishing the syndrome model and simultaneously treated with Fuzi. The treatment group was treated after the disease model was established successfully. Treatment duration was 9 days. Syndrome scores and weight were collected, and determined content level of TSH, IgG and cardiac toxicity index C3, LDH, CTn-I.Results：Fuzi water extract could improve the rat model of yang deficiency of spleen and kidney, increase spleen weight, kidney weight, adrenal gland weight and thymus weight to body weight, increase the level of serum TSH, IgG, C3 and LDH, CTn-I, and decrease heart to body weight ratio.Conclusion：Fuzi water extract has preventing and curing effect on syndrome of yang deficiency of spleen and kidney and has certain cardiac toxicity.