To explore the inhibition effects and mechanism of baicalin on LTEP-A2 cells in vitro of lung cancer patients.Methods：Human pneumonicadenocarcinoma cell line LTEP-A2 was cultured with different dose of baicalin for different periods of time. Cell proliferation was assayed by using Cell Counting Kit-8, and further in vitro study including tumor cell migration and invasion, wound healing assays were performed. Besides ,MMP-2,MMP-9,TGF-β,which were related to the migration and metastasis potential in lung cancer cell lines were detected by western blot.Results：Baicalin inhibited the proliferation of LTEP-A2 cells in a time and concentration-dependent manner. In tumor cell migration and invasion test, wound healing assays etc. showed significantly decreased migration, invasion in baicalin-treated cell groups vs control tumor cells. Further study showed that MMPs decreased after baicalin-treated. The higher doses of baicalin, the lower level of MMP-2/MMP-9.However, baicalin did not have a significant effect on the TGF-β. Conclusion：Baicalin inhibits the proliferation and migration of lung cancer LTEP-A2 cells, possibly by decreasing the expression of MMP-2, MMP-9 in part.