To explore the preventive effect of type Ⅰ bovine collagen membrane on tendon adhesion from the prospective of biomechanics. Methods： Sixty chickens at the age of 3 months， half male and half female， weighing 1.5 to 2.0 kg， were made into models with 1/2 deep tendon rupture on the third toe of the right foot. They were randomly divided into sham group， control group and type Ⅰ bovine collagen membrane group. Then four subgroups were divided according to time， including 2 w group， 4 w group， 6 w group， 8 w group. The degree of anastomosis adhesion， the toe joint angle， sliding tendons buckling distance， and tendon rupture of the biggest load were detected. Results： Two， four， six， and eight weeks after the surgery， type Ⅰ bovine collagen membrane group had better results in adhesion score compared with control group (P<0.05); the degree of anastomosis adhesion， the toe joint angle， sliding tendons buckling distance， and tendon rupture of the biggest load were better in the treatment groups (P<0.05). Conclusion： Type Ⅰ bovine collagen membrane can reduce adhesion， improve the tendon biomechanics index， and has a certain preventive adhesion effect， which is a kind of new biological material to effectively prevent tendon adhesion.