To observe the effect of electrical acupuncture in Sanyinjiao acupoint on anxiety-like behavior of natural climacterium rats. Methods：The rats were divided into normal control group， model group and treatment group. Rats in the treatment group were given electro-acupuncture at Sanyinjiao acu-point once every other day for15 sessions. Rats in the other two groups were fed with conventional methods， and received stimulation every other day. The data of anxiety behavior were recorded， including times of entering the open arm， times of rear leg upright standing in the closed arm， time entering the open arm， etc. Results： Compared with normal control group， the percentages of close-area time (OE%) and open-area time (OT%) showed an decreasing trend in model group， and the difference was significant (P<0.05， P<0.01) which showed significant symptoms of anxiety; but the difference of OE and CE between the model group and treatment group was not statistically significant (P>0.05); compared with model group， the percentages of OE and OT increased greatly， and the difference was statistically significant (P<0.05); the value of HD in model group increased greatly， and the difference was statistically significant (P<0.01)， the HD value in treatment group showed a downward trend， and the difference was significant (P<0.05)， but the RE value was not statistically significant. Conclusion： Electrical acupuncture in Sanyinjiao acupoint shows certain anti-anxiety effect.