To measure and observe pressure pain threshold in patients with chole-heart syndrome and coronary heart disease, and to find the relationship and differences between them so as to contribute to the clinical differential diagnosis of the two diseases. Methods： Pressure pain threshold of 10 relevant acupuncture points were measured on 30 patients with chole-heart syndrome, 30 patients with coronary heart disease and 30 healthy people. Results： Compared with the healthy group, the pressure pain threshold of acupuncture points related to gallbladder in chole-heart syndrome patients decreased significantly, and the difference was statistically significant(P<0.01), while the difference of acupuncture points related to heart between the two groups was not significantly different(P>0.05). Compared with the healthy group, the pressure pain threshold of acupuncture points related to heart in coronary heart disease patients decreased significantly, and the difference was statistically significant(P<0.05), while the difference of acupuncture points related to gallbladder between the two groups was not significantly different (P>0.05). Comparing chole-heart syndrome patients with coronary heart disease patients, pressure pain threshold of acupuncture points related to heart in chole-heart syndrome patients was higher, and the difference was statistically significant (P<0.01); pressure pain threshold of acupuncture points related to gallbladder in chole-heart syndrome patients was lower, and the difference was statistically significant (P<0.01). Conclusion： The study has proved the existence of differences in the two diseases in terms of their pressure pain threshold of related acupuncture points. The chole-heart syndrome patients appear hyperalgesia phenomenon in their acupuncture points related to gallbladder, and the patients with coronary heart disease appear hyperalgesia phenomenon in their acupuncture points related to heart.