To observe the effect of Modified Liang-fu Formula (MLFF) combined with 5-Fu on VEGF and bFGF expression in the serum of human gastric cancer xenografted nude mice.Methods:The animal model of SGC-7901 xenografted nude mice was established，and all the mice were randomly divided into 6 groups; namely model control group (the same amount of normal sterile saline)，5-Fu group (17 mg·d-1·kg-1)，MLFF high-dose group (10 g·d-1·kg-1)，MLFF medium-dose group (5 g·d-1·kg-1)，MLFF low-dose group (2.5 g·d-1·kg-1) and drug combination group (5 g·d-1·kg-1 MLFF combined with 17 mg·d-1·kg-1 5-Fu).After 10 days of continuous treatment，eyeball blood was withdrew，the mice were killed，and the expression of VEGF and bFGF in the serum were detected using ELISA method.Results:Compared to model control group，the expression levels of VEGF in nude mice serum were significantly reduced in 5-Fu group，MLFF high-dose group，MLFF medium-dose group，MLFF low-dose group and drug combination group，respectively (P<0.01)，while MLFF reduced VEGF expression in a dose-dependent manner.The combination group showed a lower level of VEGF compared to 5-Fu group and MLFF medium-dose group，but there was no significant difference (P<0.05).Meanwhile，compared to model control group，the expression level of bFGF in nude mice serum was significantly decreased in 5-Fu group，MLFF high-dose group，MLFF medium-dose group，MLFF low-dose group and drug combination group，respectively (P<0.01).Conclusion:MLFF can down-regulate the expression of VEGF，bFGF in the serum of human gastric cancer xenografted nude mice.It indicates that MLFF has the potential to serve as anti-angiogenesis agent for gastric cancer.