To study the drug release of temperature-sensitive nanogels of Magnolia in vitro and in vivo. Methods: We investigated the process of in vitro drug release of temperature-sensitive nanogel of Magnolia with Franz diffusion cell. The regulation of nasal absorption of temperature-sensitive nanogel of Magnolia was investigated by the rat in situ nasal deposit method. The method of HPLC was conducted. Results: The study on drug release in vitro showed that the cumulative release rate in12 hours was 14%. The treatment of dynamic model showedthat the absorption process of this preparation fitted the first-order dynamics process， with slow-release effect. Conclusion: Temperature-sensitive nanogels of Magnolia has satisfactory slow-release effect.