From the intestinal tight junction and adherens junction pathway to explore mechanism research of Rouganguchang Decoction on improving chronic alcoholic liver injury and intestinal permeability changes. Methods： The rats were given Lieber-Decarli liquid diet daily for 6 weeks to duplicate the alcoholic liver injury model. Thirty male Sprague-Dawley (SD) rats were randomly divided into control liquid diet, control group (control group, n = 10) and ethanol liquid diet model group (n = 20). Model rats were randomly divided into the model group (n = 10), Rouganguchang Decoction group (n = 10) the fourth weeks. The rats were given decoction or distilled water from the fourth week to the sixth weekend. Each rat was given 10mg/kg endotoxin (LPS) by gavage 3.5h before being sacrificed. The following indexes were detected： 1)The ALT、AST activity of serum were detected by biochemical method; 2)LPS content in the portal blood was detected by biochemical method; 3)Pathological changes in intestinal tissues were observed by electron microscope; 4)The protein and mRNA expresses of ZO-1 and Occludin were detected by Western blotting and real-time PCR respectively; 5)The protein of beta-Catenin and E-Cadherin were detected by Western blotting. Results：1) Compared with that of the model group rats, the activity of AST in drug group decreased significantly (P<0.05); 2) The plasma endotoxin levels of Rouganguchang Decoction decreased significantly compared with that of the model group rats; 3) Rouganguchang Decoction could improve the intestinal mucosa connection structure of the surface epithelial cells of model rats; 4) Compared with those of the model group rats, the protein and mRNA expressions of ZO-1 and occludin, as well as the protein expressions of beta-Catenin and E-Cadherin all increased. Conclusion： Rouganguchang Decoction can significantly improve chronic alcoholic liver injury and intestinal permeability changes, the mechanism of that was related to protection of intestinal epithelial tight junctions and adhesion junctions.