Exploring the urinary metabolic profiling of chronic hepatitis b (CHB) patients with different tongue manifestations, the red tongue with yellow tongue coating and the pale tongue with white tongue coating, to demonstrate the potential objective material basis. Methods： The urinary metabolic profiling was detected based on gas chromatography coupled to mass spectrometry (GC/MS), multivariate data was analyzed, the unsupervised segregation and supervised segregation analysis were checked, and the potential markers of interest were extracted and confirmed. Results： Distinct clustering in metabolic profiles were observed by supervised segregation analysis in different tongue groups, based on the variable of importance(VIP) in the project values, significantly changed metabolites were identified. These changes were related to the disturbance in energy metabolism, amino acid metabolism, nucleotide metabolism, and gut microflora metabolism. Conclusion： This study demonstrated that tongue manifestation may have objective material basis, and closely related to the condition of the body organs function.