To find an agent which can induce several strains of embryonic stem cells to differentiate towards germ cells,with the common inducer retinoic acid (RA) as positive control.Methods：Different strains of mouse embryonic stem cells (mESC) 1B10,D3,R1/E were respectively cultured,and induced to form embryoid bodies (EBs) which were allowed to attach; different compounds were then added to intervene the attached cells; the intervene continued for 72 h and RNA were extracted and cDNA were synthesized; finally,real-time fluorescence quantitative PCR (qPCR) assay were used to measure the transcriptional expression patterns of 11 reproductive differentiation related genes.Results：It was found that oleanolic acid (OA) can induce all 3 strains of mouse mESC to differentiate towards germ cells and the positive control RA can only induce mESC-1B10 and mESC-R1/E to differentiate towards germ cells.RA cannot induce mESC-D3 to differentiate towards germ cells.Conclusion：OA can induce several strains of embryonic stem cells to differentiate towards germ cells and it has broader universality than the positive control RA.