On the basis of the control reference drug technical requirements and the principle of equivalent homogeneity,using modern extraction and preparation technology to prepare a stable quality and good uniformity of Semen Armeniacae Amarum reference substance.By investigating the thin layer of different system,to establish the quality standards of thin layer identification with reference extract,for the use of thin layer identification in traditional Chinese medicine (TCM) standard.To investigate methodology and its main efficacy components to ensure the consistency.Methods：Different thin layer chromatographic system for Semen Armeniacae Amarum reference substance qualitative alternative was used to the reference drug research.An HPLC method for content determination of amygdalin in Semen Armeniacae Amarum reference substance was established.Results：In Semen Armeniacae Amarum reference substance in thin layer chromatography,the mobile phase was consisted of toluene- ethyl formate- methanol- formic acid and water (6∶2∶0.4∶0.1-0.1),and chromatographic corresponding to the position,the same color spots.To establish an HPLC method for content determination of amygdalin in Semen Armeniacae Amarum reference substance,the linear range of amygdalin was from 461.7 to 15.39 mg/L (r = 0.999 5),and the average recovery was 101.1% (RSD = 2.15%),The method was accurate,reliable,strong specialization and good repeatability．Conclusion：Semen Armeniacae Amarum reference substance can replace Semen Armeniacae Amarum reference drug for qualitative identification.