To study the synergistic effect of Mahuang Fuzi Xixin Decoction combined with antibiotics on single-used antibiotics, and thus to lay the experimental foundation to screen active ingredients. Methods: The ingredients of Mahuang Fuzi Xixin Decoction, the compound Chinese medicine combined cefazolin, and cefazolin were added into the tissue mediums respectively as experimental groups, a blank tissue medium was taken as a blank control group as well as the negative group, and a tissue medium added sorely with Tigecycline was taken as the positive group. Except for the blank group, inoculation of s. pneumoniae was given to each tissue medium and was cultured at 37 ℃ overnight. Every 12 hours, the mediums were taken out for recording viable counts, and minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the bacteria were detected by continuous dilution method, and inhibition zones were detected by paper slip method. Results: 1) It was found that bacteria grew gradually in the Traditional Chinese Medicine compound group at 12 h, and after cultured for 120 h, the bacteria concentration gradually decreased. Until cultured for 180 h, the bacteria concentration started to remain the same. In cefazolin group, bacteria count reduced gradually in the first 12 h, the downtrend of bacteria ended at 60 h. In Traditional Chinese Medicine compound combined cefazolin group, the bacteria count reduced gradually at the first 12 h, and after 144 h of culture, all groups of bacteria showed no sign of growth at the same time, with Tigecycline intervation bacterial count fell sharply. However, some bacteria still survive to the end of the observation. The negative groups bacteria grew slowly along culture and the bacteria of blank control group showed no sign of bacteria. 2) The Mhuang Fuzi Xixin Decoction vs. s. pneumoniae (MIC＝1.25 mg/ml, MBC=2.5mg/ml); Cefazolin vs. s. pneumoniae (MIC＝0.625 mg/ml, MBC=1.25mg/ml); Traditional Chinese medicine compound combined cefazolin vs. s. pneumoniae (MIC＝0.312 mg/ml, MBC=1.25mg/ml); Tigecycline vs. s. pneumoniae (MIC＝0.156 mg/ml, MBC=1.25mg/ml). 3) Traditional Chinese Medicine compound combined cefazolin group had close Bacteriostasis Circle diameter with the positive control group, and the second was cefazolin group. Traditional Chinese Medicine used alone had shorter Bacteriostasis Circle diameter than cefazolin group. The Mahuang Fuzi Xixin Decoction compound with cefazolin combination had enhanced antibacterial effect on streptococcus pneumoniae, and it has synergy effect (FIC＝0.59). Conclusion: The Mahuang Fuzi Xixin combined antibiotics have more obvious antibacterial effect, the reason for this might be it activates the growth of resistant bacteria.