To observe the effect of Huoxuesan on PGE2 and IL-6 level in rats with acute soft tissue injury so as to explore the possible mechanism of Huoxuesan in restoring soft tissue damage. Methods: After modeling, rats were randomized into two groups and given different drugs. Dynamic observation was conducted on the aspects of injury symptom index, histomorphology index, and serum level of PGE and IL-6 respectively, followed by statistical analysis. Results: The group used Huoxuesan performs better in injury symptom score and histomorphology score. Huoxuesan can also lower the levels of PGE2 and IL-6 in rats serum. Conclusion: Huoxuesan can diminish inflammation and swelling and relieve pain. It can also contribute to the restore of soft issue. This may due to its determent on the levels of PGE2 and IL-6.