To observe how twirling reinforcing and reducing method protect the kidney in spontaneously hypertensive rats (SHR) by recording its influence on the levels of renin and Angiotensin (AngII). Methods:Sixty males SHR were randomly divided into five groups：model control group (group A), acupuncture group (group B), acupuncture with reinforcing method group (group C), acupuncture with reducing method group (group D) and even reinforcing-reducing method group (group E). Twelve WKY rats were taken as blank group (group F). Each group adopted different acupuncture manipulations on LR3 (Tai Chong). The model and blank groups were just given acupuncture-like stimulus. After the treatment, the whole blood and renal tissues were taken from all rats, then to detect levels of renin and Ang II by ELISA and observe the pathological changes of renal tissues in light microscope. Results:Compared with the blank group, the levels of renin and AngII in blood plasma and kidney of SHR in model group were higher(P<0.01). Also, compared with reinforcing method group, reinforcing-reducing method group and acupuncture group, the results were of statistical difference(P<0.05). The levels of renin and AngII of SHR in reducing method group were significantly declined, and when compared with reinforcing method group, reinforcing-reducing method group, acupuncture group and model group, there existed statistical difference(P<0.01). No significant differences exist between reducing method group and blank group(P>0.05). Conclusion:Twirling reduction method of acupuncture can effectively reduce the levels of renin and AngⅡin blood plasma and kidney in SHR, which would explain its mechanism of kidney protection.