To investigate and determine the best formula of Tibetan medicine toothpaste and study the anti-inflammatory effects.Methods: Tibetan medicine were added as functional component.The effects of toothpaste forming process was measured by sensory property to evaluate the different ratio of glycerol,Silicon dioxide and non-ionic guar gum.Ear swelling was induced by dimethylbenzene in mice.Tibetan medicine toothpate was daubed on the ears of mice,then,the ear swelling volume was measured to analyze the anti-inflammatory effects of Tibetan medicine toothpaste.Results: The best formula was made of 3.3 g of Tibetan medicine,25 g of glycerin,40 g of sorbitol with the 70% concertration,22 g of Silicon dioxide,0.7g of non-ionic guar gum,1.1 g of oleummenthae,2.2 g of sodium dodecyl sulfate(K12),0.1 g of ethylparaben,0.05 g of propylparaben,0.35 g of saccharinand as well as proper weight of water.The Tibetan medicine toothpaste markedly suppressed the ear swelling induced by dimethylbenzene,and in contrast to Yunnan Baiyao toothpaste,the anti-inflammatory effects of Tibetan medicine toothpaste was better.Conclusion:The Tibetan medicine toothpaste is smooth and bright having a gentle mint taste,and its color is translucence dark brown and the viscosity is moderate.The Tibetan medicine toothpaste has good anti-inflammatory effect.