To illustrate the change tendency of female sex hormones during the menstrual cycle of healthy women with different personality traits.Methods: Eighty healthy women with regular menstrual cycle were recruited and evaluated by Revised Eysenck Personality Questionnaire-Short Scale for Chinese (EPQ-RSC).According to the score of different aspects in EPQ-RSC,such as Neuroticism (N),Psychoticism (P) and Extraversion (E),the subjects were divided into high group and low group.Then blood were taken from both groups during the period (day 2 to 4),the late follicular phase (day 11-13) and the late luteal phase (day 22-24) for three consecutive menstrual cycles.Estrogen (E2),follicle stimulating hormone (FSH),luteinizing hormone (LH),progesterone (Prog),testosterone (T) and prolactin (PRL) were tested.Results: On the Neuroticism there was no significant difference between the two groups (P>0.05).However,on the Psychoticism the high-P-group showed significant increasing of the FSH in the period,late follicular phase, late luteal phase and the LH in the period and late follicular phase comparing with the low-P-group ( P<0.05).There was also significant difference in the reduction of T in M3 period.On the Extraversion,the high- E-group showed significant increase of the E2 in M2 period,the Prog in M3 period,and the FSH in M1 period than the low-E-group (P<0.05).But in M3 the LH of low-E-group was clearly significantly higher than that of high-E-group.The T in M2/M3 period of the low-E-group also obviously significant higher than the high-E-group.Conclusion: Different personality characteristics may affect the change of the sex hormones of healthy women.