To investigate the influence of continuous cropping on the yield and quality of Salvia miltiorrhiza and the action of fertilization. In this paper, different kinds of fertilizers were applied to sequential cropping land and the contents of active ingredients, yield of Salvia, and antioxidant activity were investigated. The results showed that continuous cropping reduced Salvia yield by 21.78% and decreased antioxidant activity significantly, but have little effect on contents of active ingredients. Using microbial fertilizer increased yield by 21.67% and improved antioxidant activity significantly, while has no impact on contents of active ingredient. Continuous cropping reduce production and antioxidant activity, but have no effect on contents of active ingredients. Fertilization has a certain effect on reducing continuous cropping obstacle to some extend.