To explore the distribution regularities of tongue picture by collecting and analyzing muti-center nationwide tongue picture of chronic hepatitis B (ALT≥2ULN) patients. Methods： A total of 1 003 chronic hepatitis B(ALT≥2×ULN) patients were recruited. Collecting the clinical manifestations of tongue by image recording and using the“Tongue Picture Acquisition and Analysis System” which developed by Tsinghua University for data processing to analyze the Tongue Picture Feature of 1 003 chronic hepatitis B(ALT≥2×ULN) patients. Results： By analyzing the clinical data of 1 003 patients， the main tongue color of chronic hepatitis B (ALT≥2ULN) were： red， pale red， dark， and light white. The main tongue shape were： teeth prints， nourishing and moistening， swollen， tongue with ecchymosis， and blue and purple collaterals under tongue. The main coating color were： white and yellow. The main tongue coating were： thin fur， greasy fur， and thick fur. The tongue diagnosis feature of dampness-heat in the liver and gallbladder syndrome were： coating yellow， tongue red， tongue coating greasy fur， tongue shape nourishing and moistening， coating white， tongue coating thin fur， tongue coating thick fur. The tongue diagnosis feature of liver stagnation and spleen deficiency syndrome were： coating white， coating thin fur， tongue pale red， tongue red， tongue coating greasy fur， tongue shape swollen and teeth prints， tongue shape nourishing and moistening， and coating yellow. Conclusion： The pathological factors of tongue diagnosis feature of Chronic hepatitis B (ALT≥2×ULN) are mainly involved in qi deficiency， wetness hyperactivity type， and blood stasis. The tongue diagnosis feature of dampness-heat in the liver and gallbladder syndrome and liver stagnation and spleen deficiency syndrome were more consistent with the standard issued by academy， which can provide a good reference for future study.