To observe the efficacy of mecobalamin injection combined with the relaxing sinew and activating col lateral herbal medicine in the treatment of diabetic peripheral neuropathy (DPN). Methods： We divided 76 patients randomly into two groups (38 patients in each group) and observed their stable glycemic after it was under control; two groups were both given mecobalamin injection while the treatment group was also given relaxing sinew and activating col lateral herbal medicine package topical treatment. The changes in symptom scores before and after treatment， motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) and other indicators of a course of two weeks were observed. Results： The total effective rate of the treatment group was significantly higher than the control group(P<0.05). After treatment MNCV and SNCV of the treatment group significantly improved(P<0.05)， better than the control group (P<0.05， P<0.01); the integral of clinical symptom was less in treatment group than control group(P<0.01). Conclusion： The relaxing sinew and activating col lateral herbal medicine combined heat packets mecobalamin intramuscular treatment can significantly improve patient’s MNCV and SNCV and relieve the clinical symptoms， which is quick and effective.