To analyze the impact of Spleen-yang Strengthening Decoction on vascular endothelial growth factor and urinary protein excretion rate in patients with diabetic nephropathy.Methods：Seventy cases of patients with Type 2 Diabetic nephropathy who were received and treated in our hospital were randomly divided into the control group (35 cases) and the observation group (35 cases) according to admission order， both of the two groups received Diabetes Education， Diabetic diet， internal medicine oral hypoglycemic agents or subcutaneous injections of insulin (the dose were set according to blood glucose levels of the patients); the patients who combined with hypertension were added nifedipine sustained release tablets， 10～20 mg， 2 times/day. The patients who combined with hyperlipidemia were added Simvastatin， 20 mg/day. The observation group used Self-prepared kidney invigorating decoction on the basis of the control group. Both groups had 8 weeks for a course of treatment， urinary protein excretion rate (uAER) and the protein and mRNA change of VEGF in the two groups were compared before and after the treatment.Results：1) uAER improved in the two groups after treatment compared to before the treatment， the range of improvement in the observation group was more obvious (P<0.05). This illustrated that Self-prepared kidney invigorating decoction can obviously significantly improve uAER of the patients with diabetic nephropathy， inhibited the progression of diabetic nephropathy in a certain extent. 2) after treatment， protein and mRNA expression of VEGF in the two groups decreased， the downward trend in the observation group were more obvious than the control group (P<0.05)， illustrated the combine use of prescription could suppress the VEGF in the protein and gene levels. 3) uAER and VEGF levels were in abnormal distribution， correlation analysis were proceed on relevant parameters by the method of Spearman， the results found that uAER had positive correlation with the expression of VEGF.Conclusion：Spleen-yang Strengthening Decoction can reduce the serum VEGF level in patients with diabetic nephropathy， and can reduce uAER at the same time， which could possibly be the mechanism of action to protect renal function.