To establish a content determination method to simultaneously determine two kinds of chromoneS including Prim-O-glucosylcimifugin and 4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol in Fangfeng. Methods： Agilent TC-C18 (250 mm × 4.6 mm， 5 μm) was used as chromatographic column; water and methanol were used as gradient elution of the mobile phase， wave length of 254nm was detected， with the flow rate of 1 mL / min， column temperature of 35 ℃， the injection volume 10μL. Results： 4’-O-beta-Glucopyranosyl-5-O- Methylvisamminol content of Fangfeng with different growth patterns had no significant difference; there was difference in the content of Prim-O-glucosylcimifugin between wild and cultivated Fangfeng; there were differences in Chromones contents in Fangfeng with different growth patterns. Conclusion： Wild Fangfeng has better quality， and the simultaneous determination of two Chromones method is simple， accurate， with good precision， reproducibility and stability， and this can be used as determination method of Prim-O-glucosylcimifugin and 4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol in Fangfeng.