Using HPLC to determine the contents of emodin, chrysophanol, and physcion in Berchemia Lineata, which was used to produce berchemia lineata massage cream, and to compare the he extraction rates of these three components in the berchemia lineata submicron powder and slices. Methods: Using C18 column (250 mm×4.6 mm, 5μm), reverse HPLC, mobile phase: water with 0.2% formic acid, ethanol. Gradient elution system were made in this methond. Flow velocity:1.0 mL/min. Column temperature: 25 ℃. Determine wavelength: 290 nm. Results: The linear range of Emodin, chrysophanol, and physcion in berchemia lineata are 1.13～51 μg/ml (r2=0.999 9), 1.27～57 μg/ml(r2=0.999 5) and 2.22～40 μg/ml (r2=0.999 8) respectively. The standard recovery of emodin, chrysophanol, and physcion are 100.67％ with RSD 1.59%, 99.72% with RSD 1.20% and 100.29% with RSD 1.09% respectively. Conclusion: This method has high accuracy and sensitivity. Also, under the same extraction method, the contents of three components in submicron powder are obviously higher than those in the slices, meaning that ultra structure-powder technique could improve the extraction efficiency of these three components.