To explore the cold and warm property of fraction of Mori Cortex by detecting related enzyme activity in substance and energy metabolism in normal rats.Methods:SD rats were randomly divided into normal，cold medicine Coptis chinensis Franch group，Scutellaria baicalensis Georgi group，Phellodendron chinense Schneid.Group，and warm medicine Aconitum carmichaelii Debx group，Zingiber offcinale Rosc group，Zanthoxylum bungeanum Maxim.group，30% split fraction of Mori Cortex group，fatty oil fraction of Mori Cortex group.All groups were administered orally for 9 days and detected glucokinase(GCK)，phosphofructokinase(PFK-1)，phosphoglycerate kinase(PGK)，pyruvate kinase(PK)，pyruvate dehydrogenase(PDH)，acetyl coenzyme A(acetyl-CoA)，citrate synthase(CS)，isocitrate dehydrogenase(ICD)，α-ketoglutarate dehydrogenase(α-KGDHC)，fumarase(FUM)，glycogen phosphorylase(PYGL)，glycogen synthase kinase(GSK-3)，adipose triglyceride lipase(ATGL)，which related to substance metabolism in liver and heart; as well as cytochrome C reductase(CCR)，cytochrome C oxidase(COX)，ATP synthase(ATPs)，adenylate kinase(ADK)，Na+-K+ ATPase and the levels of ATP、ADP、NAD+、NADH、NAD+/NADH，which related to energy metabolism.In this way，we can explore the influence on substance and energy metabolism of the cold and warm property of fraction of Mori Cortex.Results:Comparing with the normal group，the weight of Coptis chinensis Franch group and Scutellaria baicalensis Georgi group were reduced(P<0.05 or P<0.01)and there were no difference in other groups(P>0.05).The level of GCK，PFK-1，PGK，PK，ICD，CCR，COX，ATPs，ADK were significantly decreased(P<0.05 or P<0.01)，acetyl-CoA，α-KGDHC，PYGL，ATGL were significantly increased(P<0.05 or P<0.01)，the level of ATP was decreased without significantly and the levels of ADP、NAD+、NADH、NAD+/NADH had no significant difference(P>0.05)in the groups of Coptis chinensis Franch，Scutellaria baicalensis Georgi and Phellodendron chinense Schneid.The activity of GCK，PGK，CCR，COX，ATPs，ADK，ICD were significantly increased(P<0.05 or P<0.01)and acetyl-CoA，α-KGDHC，PYGL，ATGL were significantly decreased(P<0.05 or P<0.01)，the level of ATP was increased without significantly and the levels of ADP、NAD+、NADH、NAD+/NADH had no significant difference(P>0.05)in the groups of Aconitum carmichaelii Debx，Zingiber offcinale Rosc and Zanthoxylum bungeanum.The activity of GCK、PFK-1、PGK、PK、PDH、ICD、GSK-3、CCR、COX、ATPs、ADK were significantly increased(P<0.05 or P<0.01)and α-KGDHC was significantly decreased(P<0.05)in the groups of 30% split fraction，fatty oil fraction of Mori Cortex，which showed the similar trend with cold medicine.Conclusion:The cold and warm property of Chinese traditional medicines can influence substance and energy metabolism of our body，which means the cold medicines can inhibit substance and energy metabolism and the warm medicines do the opposite，which is fit in with the new hypothesis that proposed by chief scientist Kuang Haixue professor.Overall，the groups of 30% split fraction，fatty oil fraction of Mori Cortex could inhibit substance and energy metabolism，which were similar with the cold medicines.We inferred that the property of these fractions maybe belong to the cold property.