To observe the curative effect and mechanism of Xuebijing injection in burn injury complicated with acute kidney injury.Methods:A total of 85 patients(enrolled during September 2012 to September 2015) with different degrees of burning were retrospectively analyzed, and were divided into observation group(used Xuebijing injection, 39 cases) and conventional group (unused Xuebijing injection, 49 cases). Also, 25 healthy people were set as control group. To collect urine and blood, detect urine KIM-1, NAG levels and blood urea nitrogen (BUN), and creatinine (Cr) levels in the three groups.Results:1) compared with healthy group, BUN and Cr increased significantly in patients with burning injury complicated with renal injury, (P<0.05). Before treatment, BUN and Cr were of no statistical difference in the observation group and conventional group, (P>0.05). After treatment, the downward trend of BUN and Cr in the observation group were more obvious than than of the healthy group, and the differences were with statistically significance (P<0.05). 2) after treatment, urinary KIM-1 and NAG levels were significantly decreased in the observation group and conventional group(P<0.01), and the indexes of observation group were significantly lower than that of the conventional treatment group (P<0.05); 3) urine KIM-1 were both positive correlated with BUN and Cr, and also, urine NAG had positive correlation with BUN and Cr, and urine NAG also had positive correlation with urine KIM-1.Conclusion:Xuebijing injection has an ideal curative effect for patients with burns and acute kidney injury. This might be related to its inhibition on the expression of urine NAG and KIM-1.