To observe the regulative effect of Nada Mixture on gastrointestinal motility of bile reflux gastritis(BRG) rats.Methods:SD rats were randomly divided into control group, model group, lowdose Nada Mixture group, middledose Nada Mixture group, highdose Nada Mixture group and Domperidone group. Each group has 10 rats. ELISA was used to detect SP content in gastric mucosa tissue and MTL, VIP in serum. Colorimetry was used to detect NOs content in gastric mucosa tissue.Results:Nada Mixture significantly increased the content of SP in gastric mucosa tissue and MTL in serum of BRG rats. And it significantly decreased the content of NO in gastric mucosa tissue and VIP in serum of BRG rats.Conclusion:Nada Mixture has the effect of regulating gastrointestinal hormones (SP, MTL, VIP, NO).