Mylabris is one of the most toxic Chinese medicines which belongs to Insecta Coleoptera Meloidae but was proved to have dramatically antitumor activity. The main chemical constituents of Mylabris are cantharidin,protein,amino acid and so on. This article extracted domestic and foreign literature on the topic of quality control methods of using Mylabris. This could improve the safety of using Mylabris and provide reference for clinical treatment.