To explore the effects of acupuncture at single acupoint and combined acupoints on antral interstitial cells of Cajal (ICC) in diabetic gastroparesis model rats. Methods: 50 SD male rats were randomly divided into 5 groups with 10 rats in each, namely normal group, diabetic gastroparesis model rats group (the model group), domperidone group，acupuncture at ST36 group and acupuncture at ST36+CV12 group. The rats were intraperitoneally injected with steptozotocin to set diabetic gastroparesis model. Treatments for each group lasted for 5 weeks. Numbers of ICC were detected by immunohistochemical methods. Results: The number of ICC in the model group were significantly reduced (P<0.01), compared with normal group, while numbers of ICC in domperidone group (P<0.01), acupuncture at ST36 group (P<0.05) and ST36+CV12 group (P<0.01) were improved significantly, compared with the model group. Numbers of ICC in the acupuncture at ST36+CV12 group was increased remarkably superior to the acupuncture at ST36 group (P<0.05). Conclusion:Acupuncture could improve the numbers of antral interstitial cells of Cajal in diabetic gastroparesis model rats, and acupuncture at combined acupoints was better than that of the single acupoint.