To observe the influence of Ganhuangcha cream and its influence on tumor necrosis factor-α (TNF-α), interleukin-6(IL-6) and interleukin-10(IL-10) levels in gingival crevicular fluid of patients with chronic periodontitis. Methods: A total of 82 cases of patients with chronic periodontitis were selected and divided into observation group (41 cases) and control group (41 cases) by random number table. All patients were given the routine periodontal non-surgical treatment, including supragingival scaling, subgingival scaling, scaling and root planning. The patients in the observation group were given Ganhuangcha cream filled in the periodontal pockets, and the control group were given compound iodine glycerol liquids, the treatment was performed once a week, lasting for 6 weeks. The changes in TNF-α, IL-6 and IL-10 levels in gingival crevicular fluid of patients before and after treatment were observed, and the curative effect was compared as well. Results: After treatment, the levels of TNF-α and IL-6 in the two groups were obviously declined, and the IL-10 levels were obviously increased(P<0.05 or P<0.01). Besides, the change degree of the observation group was much higher than that of the control group (P<0.05). The total clinical efficiency in the observation group(95.12%) was much higher than that of the control group(75.61%)(P<0.05). Conclusion: Ganhuangcha cream has favorable clinical effect in treating chronic periodontitis. It can reduce TNF-α and IL-6 levels in gingival crevicular fluid and increase IL-10 levels; thus controlling the inflammatory damage in periodontium effectively and improving the local inflammation..