To observe the expression of angiogenesis factor in skeletal muscle of diabetic rats with lower limbs ischemia during acupoint injection of bone marrow mesenchymal stem cells (BMSCs) with Qi-tonifying and Blood-activating Chinese medicine. Methods: Eighty Sprague Dawley (SD) rats were randomly divided into 2 groups, 10 rats in the normal blood sugar control (NMC) grooup. The other 70 rats were intraperitoneally injected Streptozotocin (50.0 mg/kg) to make DM model, then were randomly divided into 7 groups equally, namely diabetes control (DMC) group, diabetes ischemic (DMI) group, Qi-tonifying and Blood-activating (QB) group, local injection BMSCs (L-BMSCs) group, L-BMSCs + QB group, acupoint injection BMSCS (Acu-BMSCs) group, and Acu-BMSCs + QB group. Using RT-PCR method to detect the expression of VEGF and bFGF mRNA in muscular tissue. Results: The expression of VEGF mRNA on the operation side in Acu-BMSCs + QB group was higher than DMC group (P<0.05). The expression of bFGF mRNA on the operation side in DMC, L-BMSCs, Acu-BMSCs groups were higher than the uninjured side(P<0.05), and those in L-BMSCs, Acu-BMSCs + QB groups were higher than NMC, DMC, DMI groups(P<0.05). The expression of bFGF mRNA on the uninjured side in Acu-BMSCs + QB group was higher than NMC, DMC, DMI, QB, L-BMSCs groups(P<0.05). Conclusion: The expression of VEGF, bFGF were positively affected by acupoint injection of BMSCs combined with QB, which means QB Chinese herb could improve the expression of angiogenesis factor by increasing the survival rate of BMSCs injected in acupoint.