To determine the content of protein and free amino acid within the residues of the Uygur medicine. Methods: The protein and amino acid content were determined by spectrophotometry with colored by coomassie brilliant blue G-250 and inhydrin. Results: The linear range of protein determined by CBB G-250 chromogenic assay was 0.081 ~ 0.202mg, recovery was 102.06%, RSD was 0.99%; the linear range of free amino acid by Ninhydrin chromogenic assay was 0.016-0.029mg/mL, recovery was 99.83%, RSD was 1.82%. Conclusion: CBB G-250 and ninhydrin chromogenic assay is simple, rapid with high sensitivity, low cost, fine reproducible characteristics and so on.