To establish the chemical fingerprint of Microctis Folium by ultra performance liquid chromatography(UPLC).Methods:The separation was developed on ACQUITY UPLC BEH C18 column(2.1 mm×100 mm，1.7 μm)by gradient elution with acetonitrile-water containing 0.1% formic acid as mobile phase at a flow rate of 0.3 mL/min，the detection wavelength at 278 nm and column temperature at 20 ℃.The fingerprint was evaluated by similarity assay and principal component analysis(PCA).Results:Eleven characteristic peaks were established in the fingerprint，and two peaks were identified.The similarities of Microctis Folium was calculated and used for differentiating its adulterant.PCA showed that Microctis Folium and its adulterant were distinctly separated by the three most important principal components.Conclusion:The method is stable and reliable which could be applied to the authentication and quality control of Microctis folium.