To investigate the effects of the combination of Herba Epimedii and Fructus Ligustri Lucidi on lymphocyte apoptosis in the asthmatic rats with glucocorticoid intervention. Methods: Asthmatic model was duplicated and the lymphocyte apoptosis in the blood and BALF was observed by flow cytometry with the oral administration of the combination of Herba Epimedii and Fructus Ligustri Lucidi accompanied by the injection of dexamethasone for two weeks (systemic administration) and the atomization inhalation of Budesonide for four weeks (local administration). Results: Compared with the normal group, apoptosis of lymphocytes in BALF of the asthmatic rats decreased significantly (P<0.05). Both the Budesonide group and the combination group of Budesonide, Herba Epimedii and Fructus Ligustri Lucidi could increase the lymphocyte apoptosis of BALF (P<0.01). The lymphocyte apoptosis of the blood and BALF in the dexamethasone group was significantly increased (P<0.05), while reduced in the combination group of dexamethasone, Herba Epimedii and Fructus Ligustri Lucidi (P<0.05). Conclusion: The local administration of glucocorticoid combined with Herba Epimedii and Fructus Ligustri Lucidi could produce a synergistic upregulation effect on lymphocyte apoptosis, while the systemic administration of glucocorticoid combined Herba Epimedii and Fructus Ligustri Lucidi could inhibit the over-expression of lymphocyte apoptosis.