To observe the effect of activating blood and resolving stasis on injuried vascular wnt/β-catenin in carotid atherosclerosis mode rats and its possible mechanism. Methods:After one-month high fat-diet, 32 SD rats were randomly divided into sham operation group, model group, middle dose (dose in clinical equivalent) group and high dose of (2 times the amount of clinical) group. Except for the sham operation group, the other rats underwent carotid artery balloon injury surgery. After 3 days of intraperitoneally administered orally, model group and sham operation group were given equal volume of physiological saline, administered continuously for 14d. Then the expression of wnt, FzD1, AXIN1, GSK-3β, β-catenin, VEGF mRNA in injured vascular were analyzed by real-time fluorescence quantitative PCR in all groups. Results:Compared with the sham operation group, the expression of Wnt, FzD1, AXIN1, β-catenin, and VEGF mRNA levels in model group decreased(P<0.01); and after treated by activing blood and resolving stasis therapy, the expression of the above genes increased in different degrees, especially in the high dose group (P<0.01~0.05); but there was no significant difference among groups on the expression of GSK-3β mRNA. Conclusion:The theapy of activating blood and resloving stasis therapy can increase the expression of VEGF mRNA of balloon injured carotid artery vascular through up-regulating wnt/β-catenin signal pathway, which suggested that it can protect the injured vascular of carotid atherosclerosis.