To observe the effects of HuLuanKeLi on diminished ovarian reserve in rats of repeated immobilization.Methods:The adult female rats with regular cycle in model group were exposed to the scheduled daily repeated immobilization for 15 days，one time per day.The duration of immobilization was progressively increased with the subsequent episode.Daily vaginal smears were permfored to follow the estrous cycle in rats.After molding，60 rats were subdivided into 6 groups(n=10)：Model group,BuJiaLe group,TiaoJingCuYunWan group,high-dose、middle-dose and low-dose group，treated for 4 weeks respectively.After Openfield testing,blood samples were obtained from abdominalaorta，and samples of ovarian tissues were harvested.Serum levels of Estradiol(E2)，Follicle-stmiulating hormone(FSH)，Luteinizing hormone(LH)，Progesterone(PROG)and hypothalamus content of Dopamine(DA)，Norepinephrine(NA)，Serotonin(5-HT)were measured by ELISA，and the changes of wet weight and histology of ovary were observed.Results:Compared with the model group,HuLuanKeLi increased serum level of E2,PROG and decreased FSH,LH ; and decreased hypothalamus content of DA，NA，5-HT(P<0.05or P<0.01); and the wet weight of ovary increased in each dose of HuLuanKeLi groups.Conclusion:HuLuanKeLi can promote follicle growth in rats with premature ovarian failure，increase secretion of ovarian E2,PROG，and improve endocrine function of damaged ovaries.