To establish a comprehensive and precise method for quality control of Ground Beetle by the High Performance Capillary Electrophoresis(HPCE); Methods: A fused silica capillary (75 μm i.d., 21 cm effective length, 30cm total length) was used as column, borate solution was used as buffer solution (10 mmol/L，pH 9.7), inject voltage was 5 kv 10 s, separation voltage was 15 kv and detection wavelength was 214 nm, common peaks were extracted from fingerprints of different batches and origins of Ground Beetle, and content of each compound was calculated by area normalization method. Results: Total seven peaks from the fingerprint were identified, and as the content determination result indicated, content of each compound had significant differences. Conclusion: HPCE is fast, efficient, accurate and precise, which can be used as a comprehensive and systematical method for quality control of Ground Beetle.