To observe the effects of stasis-resolving and detoxificating method on the expression of thrombin and its receptor (PAR-1), monocyte chemoattractant protein (MCP-1) , nuclear factor κB (NF-κB) and infiltration of neutrophil in brain tissue of MCAO rats, and to discuss the association between thrombin and inflammatory factors and the intervention effects of stasis-resolving and detoxificating method.Methods:The MCAO rats were randomly divided into 7 groups: the sham-operated group, the model group, the blood-activating and stasis-resolving method group, the heat-clearing and detoxificating method group, the stasis-resolving and detoxificating method group, the Argatroban group and the PDTC group. And were given the drugs respectively, then the levels of thrombin and its receptor, MCP-1, NF-κB were detected after being given drugs in brain tissue of MCAO rats, and the infiltration of neutrophil in ischemic area was observed.Results:The mRNA and protein expressions of thrombin and its receptor, MCP-1, NF-κB in brain tissue of the model group increased obviously (P<0.05), while all of those decreased (P<0.05) in each treated groups after treatment, and it also present a markedly reduction in the stasis-resolving and detoxificating group. A positive linear correlation was shown in contents of protein of PAR-1, MCP-1, NF-κB and thrombin in brain tissue of each group (P<0.05). The infiltration of neutrophil in ischemic brain tissue of the model rats was visible, and there were obvious improvements in each treated groups.Conclusion:It is found that there was a correlation between thrombin toxicity and inflammation responses, and both of them were inhibited by stasis-resolving and detoxificating in MCAO rats. Furthermore, there was an obvious synergistic effect in stasis-resolving and detoxificating and heat-clearing and detoxicating method group.