To observe the effects of Naotai Formula, a qi-replenishing and blood-activating formula made up by Milkvetch Root, Sichuan Lovage Rhizome, Earthworm, Stiff Silkworm on TFR, Fpn, DMT1, FLVCR and BCRP of rats with focal cerebral ischemia．Methods:The rats were randomly divided into five groups as following：sham operation group，model group，low dose group of NTE(3 g／kg), medial dose group of NTE(9 g／kg), and large dose group of NTE(27 g／kg)． After three days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by middle cerebral artery occlusion(MCAO) with suture method．After three days，the rats were treated with previous therapy. On the third day, TFR, Fpn, DMT1, FLVCR and BCRP of rats were observed by immunohistochemical method and Western-blot.Results:The expression of the TFR and DMT1 decreased in large dose group of NTE(P<0.05); the expression of the Fpn increased in large dose group of NTE (P<0.05); the expression of the FLVCR increased in each NTE dose group(P<0.05); the expression of BCRP had no obvious change．Conclusion:Naotai formula increased expression of Fpn and FLVCR, promoted the efflux of intracellular iron, decreased expression of TFR, DMT1, and inhibited the influx of iron, regulated the iron metabolism and played a role in the protection of neurons.