To observe the effect of Jiegufang drug serum on callus osteoclasts, osteoblasts and chondrocytes in vitro growth. Methods:To culture New Zealand rabbits fracture modeling callus in vitro and prepare Jiegufang drug serum to incubate New Zealand rabbits callus. The morphological shape and the number of callus osteoclasts, osteoblasts and chondrocyte were observed. Results:After 10 d of surgery, cells began to climb out of the surrounding tissue blocks. After staining, brown granules in the cytoplasm, nucleus full and clear nucleolus could be seen. After 17 d of surgery, it can be seen that the proliferation of osteoblasts and chondrocytes grew fast and osteoclasts grew slow in the observation group. After 24 d, 31 d of surgery, osteoclasts, osteoblasts and chondrocytes grew slower. To compare with the control group, the the proliferation of osteoblasts and chondrocyte were faster than the control group after 10 d, 17 d, 24 d, 31 d of surgery. After surgery of 10 d, 17 d, 24 d, 31 d, the number of osteoclasts in the observation group was lesser than the control group, but the difference was not statistically significant(P>0.05). Compared with the control group, the number of osteoblasts and the chondrocytes in the observation group were more than the control group after 10 d, 17 d, 24 d, 31 d of surgery, and the difference was significant(P<0.05). Conclusion:Jiegufang drug serum can promote New Zealand rabbits osteoblasts and chondrocytes proliferation, and thus to promote the regeneration of bone tissue.