To explore the effect of Shegan Kangbingdu injection(SG) on inducing pseudo-allergic reaction. Methods: Animal allergy experiment was taken to explore the allergic effects on mice. MTT was used to confirm the concentration on RBL-2H3 cells. Cell degranulation effect was verified by neutral red staining experiment, and β-hexosaminidase release rate test. Fluo-3 was used to detect intracellular Ca2+ changes. The allergic reactions of SG were thus detected comprehensively. Results: SG enhanced mice auricle permeability and thus induced mice allergy; different concentrations of SG induced RBL-2H3 cell degranulation, the intracellular calciumion concentration has obviously increased due to dose-dependence Conclusion: SG can induce mice allergy, and this might related to intracellular Ca2+ increase.